| Ability360 Phoenix RisingNRAbility360 Phoenix Rising season registration: Not registered | Champions | | 0 | Phoenix, AZ |
| Ability360 UnitedNRAbility360 United season registration: Not registered | Founders | | 0 | Phoenix, AZ |
| Arizona HeatNRArizona Heat season registration: Not registered | Presidents | | 0 | Mesa, AZ |
| ASPO ForceRASPO Force season registration: Registered | Premier | Art Cartwright | 5 | Wooster, OH |
| Atlanta StingNRAtlanta Sting season registration: Not registered | Premier | | 0 | Johns Creek, GA |
| Atlanta Sting AcademyNRAtlanta Sting Academy season registration: Not registered | Founders | | 0 | Johns Creek, GA |
| Auburn UniversityRAuburn University season registration: Registered | Premier | Zachary Dickey | 9 | Auburn, AL |
| BORP BombersRBORP Bombers season registration: Registered | Non-Conference | Margo Roeckl | 8 | Berkeley, CA |
| BORP ShockersRBORP Shockers season registration: Registered | Presidents | Margo Roeckl | 9 | Berkeley, CA |
| Boston BrakersRBoston Brakers season registration: Registered | Champions | John Fecteau | 7 | Boston, MA |
| Brooks BarracudasRBrooks Barracudas season registration: Registered | Founders | Dylan Nachum | 7 | Jacksonville, FL |
| Burbank City PSPBurbank City PS season registration: Pending | Non-Conference | Armando Ornelas | 4 | Burbank, CA |
| Canada 1NRCanada 1 season registration: Not registered | International | | 0 | Canada |
| Canada 2NRCanada 2 season registration: Not registered | International | | 0 | Canada |
| Chico GophersPChico Gophers season registration: Pending | Presidents | Frank Burger | 8 | Chico, CA |
| Chippewa Valley HooligansNRChippewa Valley Hooligans season registration: Not registered | Founders | | 0 | Eau Claire, WI |
| Club Union 1 (Mexico)NRClub Union 1 (Mexico) season registration: Not registered | International | | 0 | Mex |
| Club Union 2 (Mexico)NRClub Union 2 (Mexico) season registration: Not registered | International | | 0 | Mex |
| CNY UnitedNRCNY United season registration: Not registered | Premier | Tom Cunningham | 8 | Syracuse, NY |
| Colorado Rolling RapidsRColorado Rolling Rapids season registration: Registered | Champions | Brian Broyles | 5 | Denver, CO |
| Colorado Rolling ThunderRColorado Rolling Thunder season registration: Registered | Non-Conference | Brian Broyles | 4 | Denver, CO |
| Commonwealth CruisersRCommonwealth Cruisers season registration: Registered | Founders | Kelshall Alexander | 8 | Boston, MA |
| Community Power Dragons - STLNRCommunity Power Dragons - STL season registration: Not registered | Unassigned | | 0 | Downers Grove, IL |
| Connecticut Riptide PSCRConnecticut Riptide PSC season registration: Registered | Founders | Michael Rostenberg | 6 | New Britian, Connecticut |
| DASA CyclonesRDASA Cyclones season registration: Registered | Champions | Jen Kedge | 9 | Saint Peters, MO |
| DASA FirecrackersNRDASA Firecrackers season registration: Not registered | Founders | | 0 | Saint Peters, MO |
| DASA FlamesNRDASA Flames season registration: Not registered | Unassigned | | 0 | — |
| Dreamplex DragonsNRDreamplex Dragons season registration: Not registered | Founders | | 0 | Clermont, FL |
| Eastern Mass MinutemenREastern Mass Minutemen season registration: Registered | Presidents | Sean White | 5 | Carver, MA |
| Florida SurgeRFlorida Surge season registration: Registered | Champions | Alexander Johnson | 5 | Jacksonville, FL |
| GLASA Chicago House ACRGLASA Chicago House AC season registration: Registered | Champions | David Rodriguez | 7 | Lake Forest, IL |
| Golden State DucksRGolden State Ducks season registration: Registered | Premier | Bart Stawicki | 8 | San Francisco, CA |
| Green MachineNRGreen Machine season registration: Not registered | Unassigned | | 0 | — |
| Hot ShotsRHot Shots season registration: Registered | Non-Conference | Angela Chaffee | 5 | Greenfield, IN |
| Houston BNRHouston B season registration: Not registered | Unassigned | | 0 | — |
| Houston Fireballs InfernoRHouston Fireballs Inferno season registration: Registered | Champions | Johnny Martinez | 6 | Houston, TX |
| Houston Fireballs SparkRHouston Fireballs Spark season registration: Registered | Non-Conference | | 1 | Houston, TX |
| import-ATXNRimport-ATX season registration: Not registered | Unassigned | | 0 | — |
| import-SnowstormNRimport-Snowstorm season registration: Not registered | Unassigned | | 0 | — |
| Juni SportNRJuni Sport season registration: Not registered | International | | 0 | — |
| LA Angels PFCNRLA Angels PFC season registration: Not registered | Presidents | | 0 | Los Angeles, CA |
| LA Galaxy PSCNRLA Galaxy PSC season registration: Not registered | Premier | Steve Everett | 4 | Torrance, CA |
| Lakeshore ChargeNRLakeshore Charge season registration: Not registered | Non-Conference | Jordan Criswell | 6 | Birmingham, AL |
| Lakeshore ForceNRLakeshore Force season registration: Not registered | Presidents | Lori Watkins | 5 | Birmingham, AL |
| Los Angeles Football Club (LAFC)RLos Angeles Football Club (LAFC) season registration: Registered | Champions | Jason Albaugh | 5 | Norco, CA |
| Mary Free Bed OverdriveRMary Free Bed Overdrive season registration: Registered | Champions | Pete Kaiser | 5 | Grand Rapids, MI |
| Mary Free Bed Overdrive AcademyRMary Free Bed Overdrive Academy season registration: Registered | Non-Conference | Livvie Berger | 6 | Grand Rapids, Michigan |
| Mexico National TeamNRMexico National Team season registration: Not registered | International | | 0 | — |
| Minnesota Northern LightsRMinnesota Northern Lights season registration: Registered | Premier | Kevin Larson | 6 | Minneapolis, MN |
| Minnesota ShockwaveNRMinnesota Shockwave season registration: Not registered | Premier | Tracy Mayer | 8 | Monticello, MN |
| MNUFC Power SoccerRMNUFC Power Soccer season registration: Registered | Champions | Katie Kensy | 8 | Minneapolis, MN |
| MNUFC Power Soccer AcademyRMNUFC Power Soccer Academy season registration: Registered | Founders | Katie Kensy | 8 | Minneapolis, MN |
| Motor City EaglesRMotor City Eagles season registration: Registered | Presidents | Wayne Petzke | 6 | Northville, MI |
| NEP ThundercatsRNEP Thundercats season registration: Registered | Founders | Nicole Taylor | 10 | Durham, NH |
| NEP WildcatsNRNEP Wildcats season registration: Not registered | Recreation | | 0 | Durham, NH |
| North Carolina Power SoccerNRNorth Carolina Power Soccer season registration: Not registered | Recreation | | 0 | Apex, NC |
| North Texas Fully ChargedNRNorth Texas Fully Charged season registration: Not registered | Founders | | 0 | Celina, TX |
| NoVa PatriotsNRNoVa Patriots season registration: Not registered | Non-Conference | | 0 | Fairfax, VA |
| Oceanside WhitecapsNROceanside Whitecaps season registration: Not registered | Recreation | | 0 | Oceanside, CA |
| One KnoxNROne Knox season registration: Not registered | Recreation | | 0 | Knoxville, TN |
| Platypus HellfireNRPlatypus Hellfire season registration: Not registered | Recreation | | 0 | Chesterfield, MO |
| Power Red BullsRPower Red Bulls season registration: Registered | Recreation | Gerard Harris | 3 | Fair Lawn, NJ |
| Power Red Bulls ForceRPower Red Bulls Force season registration: Registered | Recreation | Meridith Bradford | 3 | Englewood, New Jersey |
| Power Soccer PDXNRPower Soccer PDX season registration: Not registered | Unassigned | | 0 | — |
| REC4NRREC4 season registration: Not registered | Unassigned | | 0 | — |
| RHI InfernoNRRHI Inferno season registration: Not registered | Presidents | Joe Slaby | 8 | Fishers, IN |
| RHI Sudden ImpactNRRHI Sudden Impact season registration: Not registered | Premier | Jordan Dickey | 6 | Indianapolis, IN |
| Rose City RenegadesRRose City Renegades season registration: Registered | Presidents | Enoch Stalcup | 7 | Portland, OR |
| Sacramento RepublicRSacramento Republic season registration: Registered | Non-Conference | Lisa Blount | 9 | Sacramento, CA |
| San Diego Power FCNRSan Diego Power FC season registration: Not registered | Non-Conference | | 0 | Oceanside, CA |
| San Jose FlashRSan Jose Flash season registration: Registered | Premier | Chris Faria | 7 | San Jose, CA |
| San Jose SteamrollersRSan Jose Steamrollers season registration: Registered | Champions | Don Faria | 8 | San Jose, CA |
| Santa Rosa PhoenixNRSanta Rosa Phoenix season registration: Not registered | Non-Conference | Ian Kinmont | 1 | Santa Rosa, CA |
| SEASPAR SteelNRSEASPAR Steel season registration: Not registered | Non-Conference | | 0 | Downers Grove, IL |
| Shepherd StrikersRShepherd Strikers season registration: Registered | Presidents | Dustin Swafford | 10 | Atlanta, GA |
| SoCal Legacy 26NRSoCal Legacy 26 season registration: Not registered | Non-Conference | Armando Ornelas | 0 | Burbank, CA |
| SoCal VaquerosNRSoCal Vaqueros season registration: Not registered | Unassigned | | 0 | Burbank, CA |
| Sportable VipersNRSportable Vipers season registration: Not registered | Founders | JESSIE Oliff | 0 | Richmond, VA |
| STRAPS ScorpionsPSTRAPS Scorpions season registration: Pending | Premier | | 0 | San Antonio, TX |
| The Bridge Adaptive Sports & RecreationNRThe Bridge Adaptive Sports & Recreation season registration: Not registered | Recreation | | 0 | Cincinnati, Ohio |
| The North StarsNRThe North Stars season registration: Not registered | International | | 0 | — |
| Tidewater PiranhasRTidewater Piranhas season registration: Registered | Champions | Matthew Trail | 8 | Virginia Beach, VA |
| Tulsa Center TwistersNRTulsa Center Twisters season registration: Not registered | Recreation | | 0 | Tulsa, OK |
| Turnstone Fantastic FlyersNRTurnstone Fantastic Flyers season registration: Not registered | Unassigned | | 0 | — |
| Turnstone FlyersNRTurnstone Flyers season registration: Not registered | Premier | | 0 | Fort Wayne, IN |
| Turnstone Furious FlyersNRTurnstone Furious Flyers season registration: Not registered | Founders | Greg Buse | 0 | Fort Wayne, IN |
| Upper Valley WheelersNRUpper Valley Wheelers season registration: Not registered | Non-Conference | | 0 | Lebanon, NH |
| Vermont ChargersRVermont Chargers season registration: Registered | Presidents | Nate Besio | 7 | Burlington, VT |
| Wisconsin Whitetails PSCNRWisconsin Whitetails PSC season registration: Not registered | Founders | William Schultz | 9 | Madison, WI |